Silver nanoparticles promote mouse mesenchymal stem cell osteoblastic differentiation and improve bone fracture healing

R.Z. Zhang, K.K.Y. Wong
The University of Hong Kong, HK

Keywords: silver nanoparticles, mesenchymal stem cells, osteogenesis, bone fracture

Summary:

With significant ant-bacterial and anti-inflammatory properties, silver nanoparticles(AgNps) are now widely used in wound dressings. Our previous research also suggested that AgNps could promote skin wound healing through inducing epidermal stem cells proliferation. In this respect, we undertook this current study to further explore the effect of AgNps on mesenchymal stem cells (MSCs), in terms of proliferation and differentiation and their potential use in bone fracture healing. We found that firstly, AgNps could indeed promote proliferation in MSCs. In the presence of differentiation medium, AgNps could drive MSCs towards osteoblastic lineage, as confirmed by Alazarin Red and alkaline phosphatase staining. Meanwhile, in a femoral fracture model, the addition of AgNps significantly improved the healing rate of fractures when compared with controls. Our results would suggest that AgNps could promote MSCs osteoblastic differentiation and its use can improve bone fracture healing. Future clinical application may prove beneficial to our patients.