Performance Comparison of Immunosensors Modified with Polymers, Nanoparticles and Antibody

S.W. Leung, J.C.K. Lai, D. Assan
Idaho State University, US

Keywords: performance comparison, human immunoglobulin G, nanoparticle, immunosensor, antibody, electrodes


Our research group is in the quest of developing a biosensor platform that is fast in response time, versatile that can be used to detect a variety of chemicals species and metabolites, and ultra-sensitive that can detect concentrations below pico-level. In this report, we are using this biosensor platform to prepare immunosensors that can be used for detection of specific DNAs and biomarkers of cancers. When genetic defects and cancers are detected in the early stage, successful treatment and therapy are substantially higher. As a demonstration of concept, we fabricated three different immunosensors for the detection of human immunoglobulin G (HIgG) that were based on Pt, Au, and glassy carbon electrode (GCE) modified with biocomposite coatings. The biocomposite coating consisted of layers of polymers, gold nano particles, and anti-HIgG. The measurement method was cyclic-voltammetry. In general, all three immunosensors were capable of measuring HIgG at concentration levels of 10^-15 g/ml or lower. The GCE immunosensor appeared to be more superior among the three electrodes. Performance comparison of these immunosensors based on sensitivity, durability, and selectivity will be reported.