Strategy to produce low molecular weight chitosan nanoparticles for gene/vaccine therapy with controllable polidispersity

C.C. Sipoli, N. Santana, L.G. de La Torre
State University of Campinas (UNICAMP), BR

Keywords: chitosan, nanoparticles, temperature gradient, DNA


In recent years, an important research area that has been investigated extensively is gene therapy. Gene therapy allows treatment of different diseases. Chitosan is a cationic polymer obtained by deacetylation of chitin, the major compound of exoskeletons in crustaceans. The polymer is natural, non-toxic and biodegradable. The cationic characteristic of chitosan allows the DNA incorporation into the hydrogels and different parameters influence the in vivo and in vitro transfections, such as chitosan molecular weight, N/P rate and size of the nanoparticle. The main purpose of this study is produce chitosan nanoparticles using the crosslinker pentasodium tripolyphosphate (TPP) by ionic gelation using a temperature gradient to improve the polidispersity index of the final system . Chitosan low molecular weight has shown more interesting characteristics in gene therapy so was used in this work.