Poly-Vinyl Pyrrolidone (PVP)-coated Silver (Ag) Nanoparticles (Ag-PVP): a Potential to Regulate Chlamydia trachomatis Inflammation

A.N. Yilma, S.R. Singh, S. Dixit, G.G. Hidat, V.A. Dennis
Alabama State University, US

Keywords: chlamydia trachomatis, silver nanoparticles, Toll like receptor, Nod like receptor


Chlamydia trachomatis is an important cause of sexually transmitted infection that can manifest itself as either acute cervicitis, pelvic inflammatory disease, and more commonly as a chronic asymptomatic infection. Previously we reported Ag-PVP (5-10 nm) reduced major pro-inflammatory cytokines produced during C. trachomatis infection of mouse J774 macrophages.Here we tested the role of Ag-PVP on the expression level of Toll like receptor (TLR) and NOD like receptors. We employed qRT-PCR to determine the expression level of TLR2 and NOD2 using RNA extracted from C. trachomatis infected mouse macrophage cell pellets that were exposed to Ag-PVP (2.5 µg/mL). Our result shows that Ag-PVP down-regulated the expression of TLR2 and NOD2, suggesting that Ag-PVP may exert its anti-inflammatory properties by interfering with TLR2 and NOD2 expression. We next investigated the anti-inflammatory role of Ag-PVP on primary cells employing bone marrow-derived macrophages (BMDM). Ag-PVP was relatively non-toxic to BMDM (up to 6.25 µg/mL) over a 72-hour time-period. Further studies revealed, Ag-PVP (2.5 µg/mL) inhibited the production of IL-6 and CXCL10 by C. trachomatis-infected BMDM as determined by ELISA. Overall our data provides further evidence for Ag-PVP role in reducing cell surface receptors expression to regulate C. trachomatis inflammation in macrophages.