Superparamagnetic Iron Oxide Nanoparticles as Drug Delivery Vehicles

K. Vig, K. Casey, P.M. Tiwari, M. Miller, R. Samant, S.R. Singh
Alabama State University, US

Keywords: SPIONs, Docetaxel, cell viability, drug

Summary:

In recent years, nanotechnology has emerged as one of the most promising fields for biomedical applications and healthcare. The use of nanoparticles has earned much attention due to their small size, high surface area to volume ratio along with surface charge, and their optical and semiconducting properties. Superparamagnetic iron oxide nanoparticles (SPIONs), which show superparmagnetism, have been reported to be reliable and safe for many biological applications.In the present study, 10nm SPION were loaded with anticancer drug, Docetaxel. Docetaxel conjugation to SPION was confirmed by FTIR, UV-vis spectroscopy and gel electrophoresis. SPIONs uptake by cells was investigated using Prussian blue dye staining.In vitro drug release was studied which showed 55% drug release in 24 h. Drug loaded SPION were then tested on Mammary tumor cells (4T1) and showed 40% cell viability with 0.1M Docetaxel conjugated to SPION. Our results shows that Docetaxel-SPIONs can reduce the amount of required anticancer drug with reduced cytotoxicity to non-cancerous cells.