Nanohydroxyapatite modified cadaver goat-lung decellularized scaffold for bone tissue engineering applications

S.K. Gupta, A.K. Dinda, P. Potdar and N.C. Mishra
IIT Roorkee, IN

Keywords: decellularization, scaffold, osteoblast cells, nanohydroxyapatite


Goat-lung is a natural resource, cheap, easily available especially in India, and could be a starting biomaterial for scaffold fabrication, but so far no attention has been given to this tissue for its application in bone tissue engineering. The objective of the present study is to fabricate the acellular scaffold from cadaver goat-lung by using decellularization method. The resultant scaffold, after decellularization, was devoid of cells, as shown by H&E staining and DNA quantification study. The decellularized scaffold was modified with 1% (w/v) nanohydroxyapatite (nHA). HA is a major inorganic component of bone, therefore, decellularized scaffold after getting modified with nHA becomes bioactive, biodegradable and osteoconductive which can be used for bone regeneration. Scientists observed that the presence of nHA in the scaffold has strong influence on bone regeneration [1]. This is because of the fact that the use of nHA particles can incorporate nanotopographic features that mimic the nanostructure of natural bone. It was also found that the nHA particles could provide the scaffold good mechanical strength, which is prerequisite of the scaffold to be applied for bone regeneration [2].