From Viruses to Cells: Tuneable Resistive Pulse Sensors for High Resolution Characterization of Nano to Micro-Scale Particle Solutions

D. Kozak, W. Anderson, R. Vogel, M.F. Broom
Izon Science, US

Keywords: nanopore, nanoparticle, characterization, techniques, resistive pulse sensor

Summary:

Tuneable resistive pulse sensors (TRPS) have generated considerable interest for their ability to accurately characterize the size, charge and concentration of nano to micro-scale particulate suspensions. Measuring the properties of each particle (e.g. exosome, virus, microvesicle, or cell) as is passes through the elastic pore sensor provides high resolution analysis often beyond that of other analysis techniques. Furthermore, the recently developed capability to simultaneously measure the size and charge on a particle-by-particle basis provides a unique method to better characterize and understand the role that these properties play.2 Herein we describe the fundamental principles behind TRPS2 and demonstrate how it has been used to improve the characterization of particle size, charge and concentration within complex (e.g. polydisperse or multimodal size and charge) suspensions.