Supramolecular architectures of cellobiose dehydrogenase and cytochrome c on electrodes by the use of DNA as artificial matrix

S.C. Feifel, R. Ludwig, L. Gorton, F. Lisdat
Technical University of Applied Sciences, DE

Keywords: supramolecular, biosensor, enzymes, electron transfer

Summary:

The use of biological redox processes for the construction of sensors is a rather promising approach. A spotlight here is the confinement of the catalytic reaction onto the electrode surface. In this study we have been focused on the formation of bi-protein assemblies using cyt c as a redox protein, cellobiose dehydrogenase (CDH) as enzyme and DNA as second building block (artificial matrix) for the construction of new supramolecular architectures. It can be shown, that CDH can be incorporated into the protein multilayer arrangement in an active form, thus new electron transfer chain from lactose via CDH and multiple cyt c molecules toward the electrode can be constructed. In order to do so we have been applying the layer-by-layer technique with DNA for the formation of catalytically electro-active bi-protein multilayer electrodes. Furthermore we study the effect of the second building block on the formation and electron transfer properties of such architectures. It has been found, that the second building block significantly influences the layered assembly and the electron transfer chain from substrate to the electrode.