NSTI BioNano 2010

Determination of the Electroporation Threshold for pulses from 95 ns to 20 us

G. Saulis, S. Balevicius, R. Saule, V. Stankevic, N. Zurauskiene
Vytautas Magnus University, LT

Keywords: nanoporation, mouse hepatoma, electropermeabilization


The dependences of the fraction of electroporated mouse hepatoma MH-22A cells on the pulse intensity were obtained for the cells exposed to single square-wave electric pulses with the durations from 95 ns to 20 us. The amplitude of an electric pulse was varied from 0.4 to 12 kV/cm. Increasing the intensity of the electric field pulse increased the fraction of electroporated cells. The electric field which leads to a given percentage of electroporation decreased with increasing the pulse length. The dependence of the amplitude of the electric pulse required to electroporate 50% of mouse hepatoma MH-22A cells on the pulse duration was also determined.
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