NSTI BioNano 2010

Size of the Pores Created by an Electric Pulse: Microsecond vs Millisecond Pulses

G. Saulis, R. Saule
Vytautas Magnus University, LT

Keywords: electroporation, pore size, bleomycin, eryhtrocytes, mouse hepatoma cells, CHO

Abstract:

Modeling studies have revealed that the pulse of nanosecond-duration should create smaller pores than the pulse of micro-millisecond duration. It can be expected, that similar differences should also exist between the pore populations created by the pulses of micro- and millisecond-durations. Here, we compared the size of the pores created by a square-wave electric pulse with the duration of 100 us and 2 ms. For the cells studied, short 100 us-duration pulse created smaller pores than longer 2 ms-duration pulse
 
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