NSTI BioNano 2010

Electrochemiluminescent sensor for clinical analysis based on Carbon Nanotube electrodes

A. Sanginario, D. Demarchi, M. Giorcelli, M. Castellino
Politecnico di Torino, IT

Keywords: ECL, carbon nanotubes, Ruthenium, electrode, electrochemical sensor

Abstract:

Presented application is based on the use of carbon nanotubes (CNTs) for biomolecular analysis using electrochemiluminescence (ECL) detection technique. For this purpose we have grown self standing cylinder-shaped blocks of Multi wall CNTs (MWCNTs) by means of a catalytic chemical vapour deposition system, fed by gases formed in consequence of the co-evaporation of camphor (carbon precursor) and ferrocene (metal catalyst) in a nitrogen atmosphere. The blocks were subsequently back-contacted and encapsulated with polydimethylsiloxane (PDMS) as electrical insulator and sealant for their use as voltammetric electrodes. A ruthenium-complex solution was used as ECL label. The experiments show that by using MWCNTs electrodes it is possible to obtain a great increase in detection limit compared to usual Au electrodes and the reproducibility in ECL signal can be achieved.
 
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