NSTI BioNano 2010

Effect of Dynamic Environment on the Interaction between Nanoparticles and A549 Monolayer

H. Patel, H. Kim, S. Kwon
Utah State University, US

Keywords: cytotoxicity, health risk assessment, nanoparticle(s), SWCNT, dynamic culture device

Abstract:

The carbon nanotube (CNT) has been well explored for many proven applications, but very little explored to understand its potential toxic effects. It is crucial to develop viable alternatives to in vivo tests to evaluate the toxicity of engineered CNTs. Our objective is to study and characterize the molecular mechanism of single wall carbon nanotube (SWCNT)-induced cytotoxicity in a novel dynamic in vitro model, which can simulate cyclic breathing condition. Different SWCNT concentrations (5, 10, and 20 μg/ml) in combination with different exposure time (24, 48, and 72 hours) were used to simulate different exposure conditions. The levels of pro-inflammatory, oxidative stress and cytotoxic mediators were monitored, following exposure of SWCNTs with different concentration, exposure time, and size. The dynamic environment facilitates interaction between SWCNT and A549 monolayer similar as in vivo and significantly different than under static condition. The outcome of this study would help us to understand the cellular response of CNT exposure to human airway and the mechanism of progression of inhaled CNTs in the respiratory system.
 
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