NSTI BioNano 2010

Synthetic Viruses for Tissue Regenerating Bionanomaterials (invited presentation)

S-W. Lee
University of California, Berkeley, US

Keywords: phage engineering, regenerative medicine, tissue engineering

Abstract:

A fundamental challenge in bio-nanoscience is to identify an active building block that can perform highly selective functions with remarkable precision based on specific recognition, programmable self-assembly, and non-toxic biocompatibility. We have developed radically novel synthetic viruses which can control and guide cell behavior for tissue engineering materials using genetically engineered M13 bacteriophage (viruses). Filamentous M13 phage have several qualities that make them attractive candidates for use as building blocks in tissue engineering scaffolds. The M13 phage has a monodisperse, long-rod shape that enables its self-assembly into directionally ordered liquid crystalline structures. Through genetic engineering, a high-density array of peptide-based signaling molecules and therapeutic materials can simultaneously be displayed on its major and minor coat proteins. We have engineered M13 bacteriophage to display various signaling peptide that promote cell interaction (IKVAV, RGD) on all 2700 copies of major coat proteins. I will demonstrate in my seminar that such engineered phage can self assemble into directionally organized structures, which in turn dictate the alignment and direction of cell growth in 2D and 3D tissue engineering matrices. I will also introduce how to identify the novel signaling peptide using viral tissu engineering approaches. The success of our novel virus-based tissue regenerating materials will enable to manipulate cell behavior at the molecular level and regenerating various tissues, and possibly lead to the discovery of cures for challenging diseases such as spinal cord injuries.
 
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