NSTI BioNano 2010

Bacteriophage-based nanoprobe targeting pancreatic cancer in transgenic mouse model

J. Feng, S. Thomas, E. Allen, A.K. Iyer, D. Hanahan, K. Kelly, H.F. VanBrocklin, J. He
University of California San Francisco, US

Keywords: pancreatic cancer, imaging, phage, peptide, SPECT

Abstract:

Objective: Imaging probes for incipient pancreatic ductal adenocarcinoma (PDAC) would enable earlier detection and guide therapeutic development. A bacteriophage (Pan27) has been identified that homes to pancreatic cancer by virtue of displaying a peptide that binds to Plectin 1, a cytoplasmic protein translocated to the cell surface. The targeted imaging properties of the phage with the expressed peptide were assessed. Methods: The plectin-1 targeted bacteriophage (Pan27) and a non-specific control phage (HPC7) were coupled with Cy5.5 for optical imaging and with DTPA for indium-111 labeling. The phage were labeled with indium-111 for SPECT imaging. The biodistribution in both PDAC tumor mice and wild type mice, at 24 hours and 48 hours post-injection was determined. Results: The two phage were successfully labeled with indium-111 with yields of 20-30% and 50-60% for the Pan27 and HPC7, respectively. Both probes were equally taken up in the tumor tissue at 24h. Tumor uptake for Pan27 remains constant (~2% ID/g) to 48h, while HPC7 washes out (0.86%ID/g) by 48h. Tumor to non-tumor ratios increased over time for Pan27 but are constant for HPC7. The two phage nanoprobes (Pan27 and HPC7) had nearly identical pharmacokinetic properties in wild type mice, weak binding to normal pancreas, with a loss of pancreas signal from 24h to 48h, indicating no specific binding. Conclusion: A nanoprobe based on a bacteriophage (Pan27) demonstrated specific targeting to PDAC tumors in the genetically engineered mouse model. Further investigation for development of nuclear probes for early detection of pancreatic cancer is warranted.
 
Program | Symposia | Speaker Exhibitor | Press Venue Registration |
Short Courses | News | Subscribe | Contact | Site Map
© Copyright 2009 TechConnect World. All Rights Reserved.