NSTI BioNano 2010

Chip-Based Diagnostics of Cancer. Simultaneous Quantitation of Six Biomarkers

T. Ryabykh, T. Osipova, Z. Sokolova, N. Paklin
N.N. Blokhin Cancer Research Center, RAMS, RU

Keywords: cancer diagnostics, protein biochip, tumor markers, ROC-analysis, logistic regression


Tumor markers: total (PSAt) and free (PSAf) forms of prostate-specific antigen (PSA); alfa-fetoprotein (AFP); carcinoembrionic antigen (CEA); neuron-specific enolase (NSE) and human chorionic honadotropin (hCG) are the most useful for detection of widespread kinds of cancer. The biochip-based test-system permits simultaneous quantitation of all six biomarkers. The goal of the investigation is evaluation of the diagnostic performance of the biochip-based test system for simultaneous quantitation of six biomarkers. Tumor marker concentrations were determined by gel-based microchips (EIMB, RAS) by immunoassay with fluorescence detection. Sera were collected from 170 patients:108 cancer patients and 62 patients with no cancer. MedCalc statistical software was used for evaluation of area under the curve (AUC) by ROC analysis and calculations of linear and logistic regression. Linear regression analysis has revealed a high degree of correlation between the levels of each biomarker measured in biochip-based system and associated ELISA test-system (CanAg, DRG Diagnostics): correlation coefficients were 0.97 (p<0.01) for AFP; 0.95 (p<0.01) for CEA; 0.94 (p<0.01) for PSAt; 0.91 (p<0.01) for PSAf; 0.91 (p<0.01) for hCG and 0.71 (p<0.01) for NSE. Logistic regression and ROC analysis demonstrated that the diagnostic efficacy of biochip-based system for simultaneous quantitation of six tumor markers was significantly higher than efficacy of any system using one marker.
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