NSTI BioNano 2010

Chemical surface functionalization of polymeric based materials to develop nanobiosensors

M.-J. Bañuls, F.J. Ortega, M.F. Laguna, M. Holgado, C.A. Barrios, R. Casquel, F.J. Sanz, D. López-Romero, A. Maquieira, R. Puchades
Universidad Politécnica de Valencia., ES

Keywords: chemical surface modification, SU-8, photobiofunctionalization

Abstract:

Optical nanobiosensors operating in real time are finding increasing applications in biology and medicine, being one of the highest priorities in research fields in nanodevices development. One decisive step in the implementation of a nanobiosensor is the selection and optimization of the most suitable chemical functionalization for probe anchoring, allowing the desired performance of robustness, bioavailability, stability, and if necessary, spatial selectivity. To address these issues, the material nature forming the photonic structure must be taken into account. In our case, SU8 polymer on silica support. In this work, chemical functionalizations of SU-8 photoresist nanostructures are optimized and analysed. Specially, those that permit selective bioreceptor immobilization on selected sensing areas of the final device. The selectivity is achieved by using photobiotin and irradiating only on the desired zones. The insertion of photobiotin has also been included in the own fabrication process of the SU-8 photoresist and has been proven successfully on planar surfaces. In parallel, for SU-8 material, selective immobilization based on the highest adsorption of the photoresist against the silicon oxide acting as support of the nanostructures is also achieved. In this case, DL of antiBSA was 2 ng/mL
 
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