NSTI BioNano 2010

Composite Electrospun Nanofibers of Polycaprolactone and Nanohydroxyapatite for Bone Tissue Engineering Using Bone Marrow Stem Cells

G.C. Lai, Y.S. Chang, J.P. Chen
Chang Gung University, TW

Keywords: nanofibers, stem cells, nanohydroxyapatite, tissue engineering, electrospinning

Abstract:

Nanocomposites of nanohydroxyapatite (nHAP) dispersed in poly(beta-caprolactone) (PCL) were prepared by electrospinning to produce 300 nm nanofibers containing 0, 25, and 50 wt% of nHAP. The composite nanofibrous membrane (NFM) was subjected to detailed analysis for its physical and chemical properties by scanning electron microscopy (SEM), thermogravimetric analysis, x-ray diffraction, Fourier-transform infrared spectroscopy and mechanical tensile testing. Bone marrow mesenchymal stem cells (MSCs) from rabbits were seeded and cultured on the PCL/nHAP NFM scaffolds and characterized. MSCs were capable of proliferating and osteogenic differentiating in PCL/nHAP NFM scaffolds. Confocal microscopy showed that the MSCs spread and attached to the nanofibers via focal adhesions with high viability while staining for F-actin revealed the presence of stress fibers. SEM observations and alkaline phosphatase activity assays confirmed normal phenotypic osteoblast cell morphology, and time-dependent mineralization and osteogenic differentiation. Energy dispersive X-ray analysis indicated mineral Ca2+ and phosphorous deposits on cell surface and the mineralization was significantly enhanced by nHAP in nanofibers with 50 wt% nHAP giving the best result. The present study indicated that the PCL/nHAP NFM scaffolds are potential for the mineralization of MSCs for bone tissue engineering.
 
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