NSTI BioNano 2010

Multifunctional Magnetic Multi-Walled Carbon Nanotubes for Anti-Cancer Drug Delivery

A. Lu, Y.J Lai, J.P. Chen
Chang Gung University, TW

Keywords: carbon nanotube, magnetic, drug delivery, doxorubicin


Multi-walled carbon nanotubes (MWCNTs) have been identified as an effective vehicle for delivery of anticancer drugs, as they are capable of penetrating mammalian cell membranes and allow for a high drug loading due to their nanoscale dimensions and high aspect ratio. By conjugating ligand molecules which are recognized by receptors over-expressed on cancer cell surface, MWCNTs can target the therapeutic agents to the desired site with their site specific intracellular uptake and enhance the effectiveness of the treatment and reduces side effects. Iron oxide magnetic nanoparticles (MNPs) have been realized as the most popular multifunctional materials for their applications in biomedicine, which include magnetic resonance imaging (MRI) contrast enhancement, and magnetically guided drug delivery. In this work, we combine the advantages of MWCNTs and MNPs and develop a novel multifunctional magnetic MWCNTs (3M CNT) containing anti-cancer drug doxorubicin (DOX), folic acid (FA), MNP, and fluorescent dye (FITC). The proposed methodology provides dual targeted delivery of the anti-cancer drug to cancer cells under the guidance of a magnetic field and through ligand-receptor interactions while allowing real-time monitoring of drug treatment through dual-modal fluorescence and magnetic resonance imaging. Chitosan-coated MNP was first synthesized and conjugated with FA and FITC (f-FA-CS-MNP). Poly(acrylic acid) functionalized MWCNT was then synthesized through free radical polymerization and decorated with f-FA-CS-MNP through covalent bindings. The chemico-physical properties of 3M CNT were fully characterized by FTIR, TGA, XRD, TEM, and SQUID, in addition to its drug loading efficiency and drug releasing characteristics. Doxorubicin could be loaded to 3MCNT with high efficiency and enhance its cytotoxic activity toward human U87 glioma cells compared to free DOX. We confirmed that 3MCNT is efficiently taken up by cancer cells with subsequent intracellular release of DOX, which then translocates to the nucleus while the nanocarriers remain in the cytoplasm. These properties make 3MCNT a potential candidate for controlled targeted delivery of DOX for cancer treatment.
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