NSTI BioNano 2010

Novel Nano Biomaterials for Toxicity Biosensors

L. Stanciu, S. Andreescu, Y-H Won, M. Ganesana
Purdue University, US

Keywords: biosensors, hybrid materials, okadaic acid

Abstract:

The goal of this work is to investigate the fundamental parameters that play a role in the creation of new materials to be used in the design of a highly stable, sensitive, okadaic acid toxin (OA) biosensor. To achieve this goal, we investigated three advanced materials platforms based on enzyme modification of chemically active materials. The work focuses on the challenges of creating hybrid composite materials containing an enzyme involved in the mechanism of action of okadaic acid, protein phosphatase (PP2A), and model materials such as polyelectrolyte capsules, silica porous particles and hydrogels, and on the development of novel strategies that will be used to prepare these hybrid materials and construct a practical, fast and sensitive OA biosensor. The key design parameter is the combination of the high selectivity and specificity of biocatalytic processes involving PP2A, an unstable enzyme, with the unique properties of the sample matrix materials. These materials will be the foundation of new generations of platforms that would be used in the construction of biosensors of OA toxin and its derivatives. These will be applied to the evaluation of the safety of seafood products in the US and therefore contribute significantly to human health. The presented research is highly novel. To the best of our knowledge, there are very few studies that reported on OA toxin detection using hybrid materials and biosensor technology in the United States. The efficiency of different methods of enzyme immobilization for this system (i.e., covalent bonding, entrapment) was determined by analyzing their biosensing capability for the detection of the OA toxin. Screen printed electrodes (SPE) with the enzyme-hybrid materials deposited on, showed higher current response and stability than for the corresponding free enzymes. The detection limit of the OA biosensor was in the nanomolar range. The OA biosensor showed a detection limit of 0.43 µM of at a signal-to-noise ratio of 3 and a sensitivity of 265 µA/mMxcm2.
 
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