NSTI BioNano 2010

A flow-assisted point-of-care testing device based on gravitational field-flow fractionation for analysis on biological fluids

M. Mirasoli, L. Cevenini, S. Casolari, B. Roda, A. Di Carlo, P. Reschiglian, A. Roda
University of Bologna, IT

Keywords: point of care testinf, field-flow fractionation, portable, multiplexing

Abstract:

The “Point-Of-Care Testing” (POCT) approach is based on the development of portable analytical platforms suitable to perform analysis directly in any required place. A POCT device should combine portability, minimum sample pre-treatment, and the possibility to perform highly sensitive simultaneous detection of several biomarkers (multiplexing). POCT devices should also comprise an integrated module for on-line sample pre-analytical treatment and/or clean-up to achieve high sensitivity and specificity even in complex matrices. With this respect, field-flow fractionation (FFF) techniques, a family of flow-assisted separation techniques which can separate analytes based on their morphologic characteristics can be exploited. In this work, we propose the implementation of FFF as a pre-analytical module of a POCT device, thus providing a selectively enriched fraction for the analysis with an increase of overall analytical output. FFF is used to prepare whole blood samples for the automatic on-line analysis of alkaline phosphatase activity in serum. After the injection of heparin-treated whole blood in the GrFFF device, plasma is separated from cells and then directly addressed by means of a microfluidic system and valves to the analytical module. he diagnostic test gave quantitative results with low sample and reagents consumption, short analysis time and high reproducibility.
 
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