NSTI BioNano 2010

Synchrotron radiation microtomography for the ex-vivo and in-vivo evaluation of nanoparticle-labeled stem cell homing in muscular tissue

F. Fiori, A. Farini, A. Giuliani, A. Manescu, C. Villa, Y. Torrente
Polytechnic University of Marche, IT

Keywords: Duchenne muscular dystrophy, stem cells, synchrotron radiation, microtomography


This work was carried out in the frame of a more general research program aiming to use human stem cells to repair muscle damage in Duchenne Muscular Dystrophy. The results of X-ray microtomography experiments, carried out both ex-vivo and in-vivo at the European Synchrotron Radiation Facility are presented. It is shown that the distribution of intra-arterially delivered stem cells can be visualized by microtomography both in muscle biopsies and in living murine models, providing biological insights into the early processes of muscle stem cell homing.
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