NSTI BioNano 2010

Localized Cell Transfection Using Atomic Force Microscopy

J.W. Yoo, Y.J. Kim, N.J. Lee, Y.J. Choi, C.J. Kang
Myongji University, KR

Keywords: electroporation, scanning probe microscopy, cell

Abstract:

Electroporation is one of the widespread tools used to insert or inject biological substances such as DNA, RNA, proteins, drugs, and genes into a cell in molecular biology. Conventional electroporation equipment applies high electric fields (>103 V), which cause the irreparable cell damage and the depression of cell viability by the lysis cell membrane. Here we present an effective method for selective and localized electroporation of living cells using atomic force microscopy control. The cells are cultivated on the patterned electrode. This electroporation technique is applied to the adhered cells to the plate rather than to the suspended cells as used in other existing methods. Moreover, it highly enhances the cell transfection. A transfected cell was observed using fluorescence microscopy after 24 hours the electroporation process and the analysis of the transfection efficiency of human breast cancer cells (MCF7) and normal breast epithelial cells (MCF10A) were done. The atomic force microscope is a good tool for cell electroporation in that it increases the efficiency of the delivery of biological substances in cultivated conditions.
 
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