NSTI BioNano 2010

Bacteriophage tail-spike proteins as probes for specific Salmonella detection

A. Singh, J. Tanha, C. Szymanski, S. Evoy
National Institute for Nanotechnology, CA

Keywords: P22, tail-spike protein, Salmonella, Surface plasmon resonance


We report the use of genetically engineered tail-spike proteins (TSPs) of P22 bacteriophage as probes for sensitive and selective detection of Salmonella enterica serovar Typhimurium bacteria. Surface plasmon resonance technique was used to detect the host bacteria on a gold coated surface where as four strains of E. coli were used as controls. We show a selective detection of the host bacteria up to concentration as low as 10^3 cfu/ml
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