NSTI BioNano 2010

Nano-structured silicon/PLGA composite microspheres for sustained release of biomolecules

D. Fan, C. Chiappini, Y. Peng, C. Smid, X. Liu, M. Ferrari, E. Tasciotti
the University of Texas Health Science Center at Houston, US

Keywords: silicon microparticles, microemulsion, PLGA, FITC-BSA, sustained release, bone tissue engineering

Abstract:

In this study, NPSi/PLGA composite microspheres were prepared for the long-term controlled delivery of a model protein. These composite microspheres were fabricated by a solid-in-oil-in-water (S/O/W) emulsion method (Figure 1A-C). Confocal microscopy showed that multiple silicon particles were encapsulated in each PLGA microsphere (Figure 1D). The release of FITC-BSA from the composite microspheres was sustained for approximately 30 days with a small initial burst release (Figure 2). The release rate of FITC-BSA can be controlled by the particle size that can be tuned by changing the concentration of the polymer solution before microemulsion. Degradation study showed that during degradation (Figure 3) NPSi/PLGA microspheres did not significantly alter physiologic pH. A cell internalization study was performed by culturing bone marrow stem cells in the presence of NPSi/PLGA microspheres. The result showed that the microsphere could not be engulfed by the cells and did not induce any cell toxicity. In summary, successful sustained release of FITC-BSA can be achieved by this delivery platform for at least 30 days. This system, tailored for the delivery of growth factors, represents an ideal solution in the fields of drug delivery for wound healing and bone tissue engineering.
 
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